Research Projects

Role of calcineurin in glial cells in health and disease

 Calcineurin is a Ca2+/CaM dependent phosphatase that senses activity and decodes cellular messages. It is a ubiquitous enzyme and is the target of some of the most important immunosuppressants, tacrolimus and cyclosporin. While it is abundantly expressed in neurons, we have found that also glial cells express this protein. We are interested in understanding its role in norma lastroglial physiology as well as in disorders such as Alzheimer’s disease and epilepsy.

This work has been founded by PRIN (Grant PRIN-2015N4FKJ4), Cariplo Foundation (Grants 2008-2319, 2013-0795, 2014-1094)

Lim D, Mapelli L, Canonico PL, Moccia F, Genazzani AA. (2018) Neuronal Activity-Dependent Activation of Astroglial Calcineurin in Mouse Primary Hippocampal Cultures. Int J Mol Sci. 19. pii: E2997. doi: 10.3390/ijms19102997.

Tapella L, Cerruti M, Biocotino I, Stevano A, Rocchio F, Canonico PL, Grilli M, Genazzani AA, Lim D.(2018) TGF-β2 and TGF-β3 from cultured β-amyloid-treated or 3xTg-AD-derived astrocytes may mediate astrocyte-neuron communication. Eur J Neurosci. 47:211-221. doi: 10.1111/ejn.13819.

Lim D, Iyer A, Ronco V, Grolla AA, Canonico PL, Aronica E, Genazzani AA. (2013) Amyloid beta deregulates astroglial mGluR5-mediated calcium signaling via calcineurin and Nf-kB. Glia. 61:1134-45. doi: 10.1002/glia.22502.

Grolla AA, Fakhfouri G, Balzaretti G, Marcello E, Gardoni F, Canonico PL, DiLuca M, Genazzani AA, Lim D. (2013) Aβ leads to Ca²⁺ signaling alterations and transcriptional changes in glial cells. Neurobiol Aging. 34:511-22. doi: 10.1016/j.neurobiolaging.2012.05.005

Genazzani AA, Carafoli E, Guerini D. (1999) Calcineurin controls inositol 1,4,5-trisphosphate type 1 receptor expression in neurons. Proc Natl Acad Sci U S A. 96:5797-801.